模型信息
中文名称:MERS-CoV HCoV-EMC2012滴鼻感染转基因hDPP4-Tg小鼠模型
英文名称:MERS-CoV HCoV-EMC2012 intransally infection transgenic hDPP4-Tg mouse model
类型:MERS-CoV感染动物模型
分级:NA
用途:用于MERS-CoV感染研究。
研制单位:病原微生物生物安全较好实验室@@http://www.natureindex.com/institution-outputs/china/state-key-laboratory-of-pathogen-and-biosecurity-amms/55010c6f140ba079548b4567
保存单位:病原微生物生物安全较好实验室@@http://www.natureindex.com/institution-outputs/china/state-key-laboratory-of-pathogen-and-biosecurity-amms/55010c6f140ba079548b4567
研究背景
暂无介绍
制备方法
Six-week-old female hDPP4-Tg mice27 were maintained in a pathogen-free facility and housed in cages containing sterilized feed and drinking water.r. MERS-CoV (HCoVEMC2012 strain) was propagated and titrated on Vero cells. Following intraperitoneal anesthetization with sodium pentobarbital (5 mg/kg of body weight), mice were treated intravenously (600 μg/kg, i.v.) with a monoclonal Ab (mAb) against mouse C5aR (Hycult Biotech,The Netherlands) or phosphate-buffered saline (PBS) as a sham treatment control. All mice were then intranasally inoculated with MERS-CoV (103.3 50% tissue cultureinfectious dose (TCID50)) in 20 μl Dulbecco’s modified Eagle’s medium.
评价验证
暂无介绍
生物安全性
暂无介绍
讨论与结论
暂无介绍
了解中析
实验室仪器
合作客户
